Coding

Part:BBa_K4806002:Design

Designed by: Luca Langenberg   Group: iGEM23_RPTU-Kaiserslautern   (2023-09-13)


CYPCamC gene for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 281
    Illegal PstI site found at 553
    Illegal PstI site found at 1277
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 281
    Illegal PstI site found at 553
    Illegal PstI site found at 1277
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 281
    Illegal PstI site found at 553
    Illegal PstI site found at 1277
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 281
    Illegal PstI site found at 553
    Illegal PstI site found at 1277
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo Parts.
We had to insert a few mutations to reduce the amount of cytosine, improving the synthesis of our DNA. Additionally, we had to insert mutations to remove recognition sites of the restriction enzymes BbsI and BsaI (the MoClo standard of our lab is BbsI instead of SapI).
Further, we had to incorporate introns because Chlamydomonas reinhardtii is an eukaryotic organism.

Source

https://www.uniprot.org/uniprotkb/P00183/entry

References